In article <31CE99F0.1EDA at unity.ncsu.edu>, Susan Jane Hogarth
<sjhogart at unity.ncsu.edu> wrote:
> I'm going to be doing some flow cytometry on worm cells and am looking
> for a good nuclear isolation protocol. I've looked through the worm
> literature index, but can't seem to come up with anything - either I'm
> using the wrong search terms or it's not there or... any ideas? Thanks.
Our lab use to do nuclei isolations quite routinely. Check out DNA and
Cell Biology 9, 177-191 (1990). The protocol should be easy to follow
from the paper, but we could dig out our full lab protocol if needed.
Hope this helps.