Egg white lysozyme is a good experiment. Raw product is found in the
grocery store. Simple homogenization, isoelectric precipitation, ion
exchange chromatography, lyophilization and characterization. Substrate is
a strain of bacteria. Then just measure activity on a spectrophotometer.
In article <wightman-1006961026470001 at garriga4.berkeley.edu>,
wightman at mendel.berkeley.edu wrote:
> I was wondering if anyone could provide suggestions for good proteins to
> partially purify/purify for an introductory biochemistry lab for
> undergraduates. I'd like to be able to give students an experience with
> purification by gel filtration, ion exchange, and/or affinity
> chromatography. Preferably, the protein should have an activity that can
> be assayed easily and cheaply. The starting material should be something
> that's clearly recognizable as "life". Unfortunately, the number of steps
> has to be very limited, as time does not allow anything particularly
> complicated. Also, it has to be doable at room temperature.
>> Any ideas, good references, or protocols?
> Bruce Wightman
> Department of Molecular and Cell Biology
> 401 Barker Hall- Garriga Lab
> University of California
> Berkeley, CA 94720-3204