Subject: Time:12:12 PM
OFFICE MEMO Injecting phage DNA Date:4/12/95
I am trying to do transformation rescue experiments using phage DNA. Are there
any tricks to this? I would especially like to know whether certain phage prep
protocols are better for injection than others (I have prepped from liquid
lysates using a PEG precipitation followed by phenol chloroform extractions and
EtOH precipitation). Also, how much DNA is usually injected?
All comments are welcome. I will post a summary as soon as possible.
Laura Selfors (Michael Stern's lab)