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Training course:Epitope tagging: a method of study for proteins

tonu tmargus at ebc.ee
Fri Jul 6 15:42:43 EST 2001

Training course:
Epitope tagging: a method of study for proteins

Dates: August 26 - September 2, 2001
The closing date for registration is August 1st, 2001

Mart Ustav, Prof Head of the Department of Virology, University of 
Tartu, Estonia
Ain Laving, Deputy Director,      Estonian Biocentre, Tartu, Estonia
Tiit Talpsep, Director of Sales and Development, Quattromed Ltd, Tartu, 
Anne Nööp, Project Manager, Quattromed Ltd, Tartu, Estonia

Epitope tagging provides a simple strategy for detecting, characterizing 
and purifying proteins. It is a recombinant DNA method in which a 
protein encoded by a cloned gene is made immunoreactive to a known 
antibody. This process is accomplished by inserting an epitope tag, a 
foreign amino acid sequence into a protein sequence. The new modified 
protein with the tag is now called the epitope tagged protein. 
Pre-existing antibodies that recognize the tags on proteins are able to 
track the function, movement, interaction and modification of the tagged 
protein in vivo and in vitro. The epitope-specific antibodies can be use 
to purify the tagged proteins.

This event is a practical training course on the use of epitope tagging 
approach in postgenomic studies. The course will demonstrate the use of 
His-tag and a new E2-tag (will be on market at September, 2001). The 
basic methods like cloning, transfection, detection (Western blot, 
immunostaining, co-immunoprecipitation), protein purification will be 

Practical training and lectures will be held at the Quattromed Ltd and 
the Estonian Biocentre, Tartu, Estonia.

Participants on the practical course will be accomodated at nearby hotel 
in Tartu.

August 26 - September 2, 2001

Speakers and instructors:
Jurjen Balkema	Amersham Pharmacia Biotech, Helsinki
Ari Jouppila		Amersham Pharmacia Biotech, Helsinki
Rein Sikut		FIT Biotech, Tampere-Tartu
Mart Ustav		University of Tartu
Reet Kurg		University of Tartu
Urve Toots		University of Tartu
Andres Männik	University of Tartu
Jüri Parik		University of Tartu
Tiit Talpsep		Quattromed Ltd, Tartu

12 participitants are invited to register for the course

Please fill in the form  (look form homeage , registration 
http://www.5dvision.ee/tagging/) and submit or send your application by 
fax: 372 7 380 284
Persons applying to attend the practical course will have to pay for 
their own travel to Tartu.
Accomodation, meals and registration fee will be covered by the organizers.

Travel information:
Visas to enter Estonia are not required from citizens of most European 
countries. For detailed information please visit the website of Estonian 
ministry of Foreign Affairs (www.vm.ee).
The closest airport is located in Tallinn, the capital of Estonia. The 
distance between Tartu and Tallinn is 186 km. Direct flights to Tallinn 
are available from Helsinki, Stockholm, Copenhagen, Amsterdam, 
Frankfurt, Vienna, London and several other cities in Europe.
  Ferries and boats to Tallinn port are from Helsinki and Stockholm.

For further information, please contact
Tiit Talpsep  E-mail ttalpsep at quattromed.ee

The closing date for registration is August 1st, 2001.

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