Epitope tagging: a method of study for proteins
Dates: August 26 - September 2, 2001
The closing date for registration is August 1st, 2001
Mart Ustav, Prof Head of the Department of Virology, University of
Ain Laving, Deputy Director, Estonian Biocentre, Tartu, Estonia
Tiit Talpsep, Director of Sales and Development, Quattromed Ltd, Tartu,
Anne Nööp, Project Manager, Quattromed Ltd, Tartu, Estonia
Epitope tagging provides a simple strategy for detecting, characterizing
and purifying proteins. It is a recombinant DNA method in which a
protein encoded by a cloned gene is made immunoreactive to a known
antibody. This process is accomplished by inserting an epitope tag, a
foreign amino acid sequence into a protein sequence. The new modified
protein with the tag is now called the epitope tagged protein.
Pre-existing antibodies that recognize the tags on proteins are able to
track the function, movement, interaction and modification of the tagged
protein in vivo and in vitro. The epitope-specific antibodies can be use
to purify the tagged proteins.
This event is a practical training course on the use of epitope tagging
approach in postgenomic studies. The course will demonstrate the use of
His-tag and a new E2-tag (will be on market at September, 2001). The
basic methods like cloning, transfection, detection (Western blot,
immunostaining, co-immunoprecipitation), protein purification will be
Practical training and lectures will be held at the Quattromed Ltd and
the Estonian Biocentre, Tartu, Estonia.
Participants on the practical course will be accomodated at nearby hotel
August 26 - September 2, 2001
Speakers and instructors:
Jurjen Balkema Amersham Pharmacia Biotech, Helsinki
Ari Jouppila Amersham Pharmacia Biotech, Helsinki
Rein Sikut FIT Biotech, Tampere-Tartu
Mart Ustav University of Tartu
Reet Kurg University of Tartu
Urve Toots University of Tartu
Andres Männik University of Tartu
Jüri Parik University of Tartu
Tiit Talpsep Quattromed Ltd, Tartu
12 participitants are invited to register for the course
Please fill in the form (look form homeage , registration
http://www.5dvision.ee/tagging/) and submit or send your application by
fax: 372 7 380 284
Persons applying to attend the practical course will have to pay for
their own travel to Tartu.
Accomodation, meals and registration fee will be covered by the organizers.
Visas to enter Estonia are not required from citizens of most European
countries. For detailed information please visit the website of Estonian
ministry of Foreign Affairs (www.vm.ee).
The closest airport is located in Tallinn, the capital of Estonia. The
distance between Tartu and Tallinn is 186 km. Direct flights to Tallinn
are available from Helsinki, Stockholm, Copenhagen, Amsterdam,
Frankfurt, Vienna, London and several other cities in Europe.
Ferries and boats to Tallinn port are from Helsinki and Stockholm.
For further information, please contact
Tiit Talpsep E-mail ttalpsep at quattromed.ee
The closing date for registration is August 1st, 2001.