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Carolina Workshop on "Production of Monoclonal Antibodies"

R. Wayne Litaker fleurs at cahaba.med.unc.edu
Sat Apr 6 18:53:43 EST 1996

Carolina Workshop on "Production of Monoclonal Antibodies"

University of North Carolina at Chapel Hill

June 16 -- 21, 1996

Lectures and laboratory exercises will cover the production, identification,
and isolation of monoclonal antibodies employing the standard Kohler and
Milstein polyethylene glycol induced methodology. Participants will be
instructed to perform basic tissue culturing techniques, including hybridoma
propagation, cryopreservation and subsequent recovery from long term liquid
N2 storage. Instruction will include small animal techniques covering
immunizations, blood serum collection, and splenocytes preparation. Each
participant will perform a murine fusion, and will utilize antibody capture
ELISA assays, dot blots, electrophoresis and Western blotting, as well as
methods for detecting cell surface antigen-specific antibody.  Techniques
in lymph node fusions, immunocytochemistry, DNA-based immunization, cloning
by limiting dilution for the isolation of monoclonal cell lines, isotyping,
production and purification of antibody, and ascites production will be


Tuition is $950.00
Deadline - May 17, 1996
Instructors: Katherine E. Kilpatrick and Debra Weige, Glaxo Wellcome
                         Steve Oglesby and Kara Hiller, UNC-Chapel Hill

CAROLINA WORKSHOPS are a series of intensive hands-on laboratory course
designed to teach cutting edge methods.  Many applicants to the Carolina
Workshops already hold the M.D. or Ph.D. degree.  Our courses are heavy
on hands-on contact with the protocols, light on lectures concerning the
basics.  Applicants unfamiliar with the theoretical basis of monoclonal
antibody production may profit from reading a text such as Antibodies: A
Laboratory Manual, Harlow and Lane, (1988) Cold Spring Harbor Press.
Twelve years experience with running these courses indicates that both
students with and without any prior hands-on experience with molecular
techniques find these courses useful.  Experienced students benefit from
in-depth interaction with the instructors; no vices learn the realities
behind the innocent looking protocols.

TO APPLY, send a curriculum vitae and a brief letter describing your
research interests.  Applicants should contact the program office as soon
as possible.  Please indicate your complete mailing address and
telephone/fax number.  Full consideration will be given to applications
received by May 17, 1996

For further information or to apply, contact:
Dr. Wayne Litaker, Facility Director
University of North Carolina at Chapel Hill
Program in Molecular Biology & Biotechnology
402 Taylor Hall CB 7100
Chapel Hill, North Carolina  27599-7100
TELEPHONE: (919) 966-1730,  FAX (919) 966-6821
Litaker at med.unc.edu

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