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Carolina Worlshop on Gene Targeting and Transgenic Mice

R. Wayne Litaker fleurs at cahaba.med.unc.edu
Sat Apr 6 18:54:08 EST 1996


July 28 -- August 9, 1996

University of North Carolina at Chapel Hill

Students will be introduced to the mouse as a developmental system, and
will learn molecular genetic manipulations of this mammal. Specific
techniques include embryonic stem (ES) cell culture, electroporation and
selection, Cell aggregation, embryo isolation and microinjection, embryo
culture and transfer, oviduct and uterine transfer of embryos, vasectomies,
and expression analysis.  These techniques are the basis for generation
and analysis of mutant mice via microinjection and embryonic stem cell
manipulation and transfer.  We will discuss the uses of these technologies
in studies of mammalian development and in generating animal models of
disease.  A seminar series on the application of gene targeting and
transgenics in various research areas will run concurrently with the course.

Tuition is $2,500
Deadline-June 15, 1996


Sarah Bronson, University of North Carolina at Chapel Hill
John Hagaman, University of North Carolina at Chapel Hill
Nobuyo Maeda, University of North Carolina at Chapel Hill
Kathy Mohr, University of North Carolina at Chapel Hill
Andrew Reaume, Cephalon

CAROLINA WORKSHOPS are intensive hands-on courses designed to teach cutting
edge methods in molecular biology and biotechnology.  Four or five courses
on different topics in molecular biology and/or biotechnology are offered
each year.  Most participants in the Carolina Workshops already hold M.D.
or Ph.D. degrees, or are advanced pre-doctoral students.  The courses are
designed for novice students as well as for individuals with prior experience.
All students benefit from in-depth interaction with instructors.

Applicants should be familiar with the theoretical basis and general
techniques of molecular biology.  Manipulating the Mouse Embryo by B. Hogan,
et. al. (Cold Spring Harbor Laboratory, 1994) and Gene Targeting:
A Practical Approach by A.L. Joyner, (Oxford University Press, 1993) both
provide an excellent introduction to mouse genetics and techniques covered
in the course.  Previous experience running this course indicates that both
students with and without any prior hands-on experience with tissue culture
and mouse genetics will find the course useful.

TO APPLY, send a curriculum vitae and a brief letter describing your research
interests.  Applicants should contact the program office as soon as possible.
Please indicate your complete mailing address and telephone/fax number.  Full
consideration will be given to applications received by June 15, 1996.

For further information or to apply, contact:
Dr. Wayne Litaker, Facility Director
University of North Carolina at Chapel Hill
Program in Molecular Biology & Biotechnology
402 Taylor Hall CB 7100
Chapel Hill, North Carolina  27599-7100
TELEPHONE: (919) 966-1730,  FAX (919) 966-6821
Email Address: litaker at med.unc.edu

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