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Automated/Diagnostic DNA sequencing Workshop

Winston Hide whide at hen.imgen.bcm.tmc.edu
Thu Sep 24 11:31:24 EST 1992


                                   October 29-31, 1992
                                Baylor College of Medicine
                             Institute for Molecular Genetics
                                  Houston, Texas 77030

Workshop description:

The generation of high quality sequence data has become an essential
tool in molecular biology and diagnostics.  The objective of the
workshop is to provide exposure to the techniques, protocols and
instrumentation currently utilized in the field of automated fluorescent
DNA sequencing.  The workshop will focus upon the areas of high-
throughput, large-scale DNA sequencing and PCR based diagnostic
sequencing.  Automated DNA sequencers (ABI and Pharmacia) as well
as robotic workstations for sequencing and PCR automation (ABI,
Beckman and Perkin Elmer Cetus) will be discussed, demonstrated
and utilized.

The following issues (lab techniques/protocols) will be covered in
discussion, demonstrations and hands-on laboratory work:

I       Template Preparation

        a.      Single strand template preparation by the glass fiber filter
        b.      Semi-automated PCR template preparation for large-scale
        c.      PCR template preparation for mutation detection and HIV
                characterization utilizing derivitized/biotinylated PCR
                primers for solid phase sequencing.

II      Sequencing

        a.      Automated cycle-sequencing performed on robotic
        b.      Closure strategies involved in large-scale sequencing
                projects and dye terminator sequencing.
        c.      Solid phase sequencing for mutation detection and

III     Examination of Sequence Data

        a.      Error and accuracy
        b.      Diagnostic sequencing for mutation and heterozygote
        c.      Troubleshooting DNA sequencing (chemistry and

IV      Sequence Assembly

        a.      Overview of available programs for transferring, editing
                and assembling data for large-scale sequencing projects.

For further information contact:
Lori Lawler-Lopez, Administrative Coordinator
Telephone:      713-798-5393
FAX:            713-798-5741
email:  llawler at bcm.tmc.edu

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