Hi everybody,
It would be extremely helpful if some one can answer the following questions
for me.
1. What is the largest commercially available Single Stranded DNA
(that's much longer than the M13 (7249 bp) DNA)
2. What are the denaturing agents that can be used to denature a
double strand (e.g. Lambda DNA) to get a single strand, preserving
streptavidin-biotin bonds? Also what's the ideal pH and salt concentration
to keep the single strand intact after the double stranded DNA was
denatured?
Looking forward to your replies.
Thanks in advance.
Shankar
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