On 11 Dec 2003 22:17:25 -0000, zy2012 at columbia.edu wrote:
>Thank you very much. I agree that apoptosis is specific style of
>death and there are many hallmarks in the apoptotic cell. My
>question is that after concentration of drug which should not
>induce apoptosis is enough to kill cell, there must be
>phosphatidyserine exposure on the outer member leaflet and DNA
>fragment because of the release of nucleases. It seemed to be
>confirmed by my experiment. For example, puromycin can kill
>leukemia cell line, and it should not induce apoptosis of the cell
>line. However, the experiment showed that there was clear DNA
>fragment and positive result with phosphatidyserine exposure on
>the outer member leaflet after puromycine treatment. Maybe,
>puromycin can induce the apoptosis of the cell ? And is it
>necessary to identify the two kind of apoptosis ?
>
Have you done a literature search on
puromycin AND apoptosis
? Search Medline using that pair of search terms, and you will get
some info.
bob
