I break down 64 codons into 16 head dinucleotides and 16 back dinucleotides
to abridge Kazusa Codon Usage Table (http://www.kazusa.or.jp/codon/)
Occurrence frequency of dinucleotides GA is maximum at head of codon
and is minimum at back of codon , on the other hand dinucleotides
TG is minimum at head and maximum at tail for most typical species from Homo
sapiens to E. coli .
Therefore correct open reading frame will have maximum (GA-TG) content in si
x reading frames.
I think this GA and TG characteristics are more useful than GC content
to find correct open reading frame and gene.
Please refer to my home page in detail http:// www1.ocn.ne.jp/~adachirh/.
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Osaka Science & Technology Center
Riichi Adachi
adachi at rsp.ostec.or.jp
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