IUBio

Incubation buffer

Pedro zanotta at unb.br
Tue May 9 12:54:40 EST 2000


I'd make 10 mM citrate, since it seems to be a combination of the two
buffers.
Hope this help!
Pedro

Tim Simmons wrote:
> 
>  Sorry for asking what is probably a very boring and basic question,
> but........
> 
>  How do I go about preparing what is described as a "10mM sodium
> phosphate-citrate buffer pH 6.0" I can make the 10mM sodiun phosphate,
> but what type and how much "citrate" do I add?  I assume the pH has to
> be adjusted afterwards to 6.
> 
>  Cheers
> Andy
> ---
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