In article <38156025.1.5643 at mx1-11.onmedia.com>, "KevinKao"
<KevinKao at goplay.com> wrote:
> Hi, I am encountering a problem trying to deduce the 5' end sequence
> of a parainfluenze virus. I have tried the Clonetech kit and didn't
> obtained a good result. Then I checked the published sequence of PIV
> and found that there might be some secondary sturctures that could
> interfere with my RT reaction. I have also tried the T4 RNA ligase
> method with a short piece of DNA oligo (I am still waiting on my RNA
> oligo) but it didn't work possibly due to the low efficiency of the
> RNA ligase working with a DNA oligo.
>> So my question is that has anybody out there who can give me some
> pointer on how they did it??? Or some information on where I could
> find some good literature??? Thanks
>> Kevin
bionet.general is dead as far as bench science is concerned.
Try posting to bionet.molbio.methds-reagnts (hopefully my
newsreader will have allowed me to to this for you).
Sorry I can't help you myself but I didn't want to leave
you stranded.
Bernard
--
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA
