This notice contains information about the next meetings for LRIG Mid
Atlantic, LRIG San Diego, LRIG SouthEast, LRIG Bay Area, and LRIG New
England.
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The Laboratory Robotics Interest Group Mid Atlantic Chapter
December 1999 Meeting
High Throughput Screening Using the 1536-Well Format:
Distinguishing Fact from Fiction
Date: Tuesday, December 7, 1999
Place: Hanover Marriott,1401 Rt 10 E, Whippany, NJ 07981
Phone: 973-538-8811, Fax: 973-538-0291
Itinerary: Social Period & Exhibition - 3:30 to 6:30 PM
Presentations - 6:30 to 8:00 PM
Panel Discussion - 8:00 to 9:00 PM
Registration: Requested, not required. Pre-registering will allow us to more
accurately gauge seating requirements and refreshment needs. Indicate names
of attendees and company affiliation. Pre-register by email with
<mailto:andy.zaayenga at lab-robotics.org> or by phone at (732)302-1038. In
order to speed sign-in at the meeting, please bring a business card to drop
into the registration box. There will be a business card drawing for a
PalmPilot IIIx donated by TekCel, www.tekcel.com.
Agenda:
There will be a variety of vendors available exhibiting the latest
developments in laboratory automation hardware and software related to
1536-well and higher density formats during a social period (3:30 P.M. -
6:30 P.M.). Food and refreshments will be available, provided by LJL
Biosystems, www.ljlbio.com.
This will be followed by three talks and a panel discussion. Members
interested in presenting a poster are encouraged to do so. Open career
positions at your company may be announced or posted. There is no
registration or fee associated with this LRIG function.
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Exhibitors:
Amersham Pharmacia Biotech
Beckman Coulter
Becton Dickinson
BioSignal
Cardinal Instrument
Cellomics
Corning
CRS
EMAX Solution Partners
Genevac
Greiner America
IDBS
LabVantage Solutions
LJL BioSystems
Marsh
MJ Research, Inc.
Modern Drug Discovery
Molecular Devices
Nalge Nunc International
Oyster Bay Pump Works
Packard
Perkin Elmer Biosystems (Tropix division)
Perkin Elmer Wallac
Pierce Chemical
Popper and Sons
Robbins
Robocon
Tecan
Tomtec
Zymark
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Presentation: The Complete Solution to ultra-High Throughput Screening Assay
Miniaturization.
David A. Dunn; Senior Research Fellow; Pharmacopeia, Inc.
A complete solution to the challenges of assay development in miniaturized
format, including plate miniaturization, liquid handling, optical detection,
data management and assay development, is required in order to implement a
viable uHTS program. This talk will cover new tools, developed at
Pharmacopeia and elsewhere, that address these challenges. Examples of their
successful implementation as part of our regular screening operation will be
given.
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Presentation: An Ultra High-Throughput Approach for an Adenine Transferase
Using Fluorescence Polarization
Dr. Julie Li, Hoechst Marion Roussel
We have developed a novel assay for measuring the activity of an enzyme that
transfers multiple adenine-containing groups to an acceptor protein. The
assay is based on fluorescence polarization (FP) technology in a 1536-well
plate format. In the assay, a long wavelength fluorescence tracer, Texas Red
(Rhodamine), was covalently conjugated to adenine of the donor substrate
through a C6 spacer arm. As a result of the transfer of the
adenine-containing moieties to the acceptor protein substrate, the rotation
correlation time of the Texas Red conjugate increased, and hence the degree
of fluorescence polarization. The pharmacological profile and kinetics of
the enzyme measured according to the FP method were consistent with those
determined previously by conventional analysis. We have successfully
executed a 250,000 compound high throughput screening program based on the
FP assay method. The quality and validity of the assay were verified by a
variety of statistical analyses.
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Presentation: Analysis of Protein-Peptide Interaction by a Miniaturized
Fluorescence Polarization Assay Using Cyclin Dependent Kinase 2/Cyclin E as
a Model System
Ilona Kariv, Sokhom S. Pin, and Kevin R. Oldenburg
Leads Discovery, DuPont Pharmaceuticals, Wilmington, DE 19880
As a result of the increasing size of chemical libraries, more rapid and
highly sensitive strategies are needed to accelerate the process of drug
discovery without increasing the cost. One means of accomplishing this is to
miniaturize the assays that enter high throughput screening (HTS).
Miniaturization requires an assay design that has few steps, has a large
degree of separation between the signal and background, and has a low well
to well signal variation.
Fluorescence polarization (FP) is an assay type that, in many cases, meets
all of the above requirements. FP is a homogenous method that allows
interactions between molecules to be measured directly in solution. This
article demonstrates the application of FP to a miniaturized HTS format,
using 1536-well plates, to measure direct binding between cyclin dependent
kinase 2/cyclin E complex (CDK2/E) and an 8-mer-peptide kinase inhibitor.
The data indicate that low variability and high specificity allow rapid and
precise identification of antagonist compounds affecting CDK2/E-peptide
interactions.
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Panel: Following the talks, there will be a moderated panel discussion with
HTS experts who are using 1536-well and higher density formats in their
laboratories.
Panel members include:
David A. Dunn, Senior Research Fellow; Pharmacopeia
Dr. Julie Li, Hoechst Marion Roussel
Ilona Kariv, Leads Discovery, DuPont Pharmaceuticals
Daniel Chelsky, BioSignal
Jonathan Burbaum, Associate Director, Technology Business Development,
Pharmacopeia
Meng Zhang, Applications Scientist, LJL
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Poster: Fluorescence Detection Strategies for Electroseparations in Plastic
Micro-fabricated Devices
Shau-Chun Paul Wang, Department of Chemistry, University of Michigan,
scwang at umich.edu
Fluorescence background interference from the device is inherent in plastic
microchips, particularly with blue or UV excitation. Conventionally,
microchip background has been reduced with confocal optics or circumvented
with specialized long wavelength fluorophores. We show that microchip
background can be rejected with analyte velocity modulation. In this scheme
the driving voltage is modulated at low frequency. Migration velocities and
analyte signals are modulated at the same frequency. Microchip fluorescence
is unmodulated, so that lock-in detection (synchronous demodulation) easily
separates the analyte signal from background. The technique does not require
a laser source. In our implementation a blue (485nm) LED is the light
source. Simple optics are used to shape the source and focus it to a spot
about 50 microns in diameter inside a microchip. Photomultiplier detection
is employed and a lock-in amplifier is used to demodulate the signal.
Apertures in the system generate a derivative response, which can be
converted to conventional bands by integration. Fluorescence rejection
provided by our first generation system lowers detection limits by five to
eight fold compared to DC measurements with the same optical train.
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Poster: Corning 1536-Well Assay Plate for HTS
Arthur Trombley, Corning Inc. Science Products Division, Portsmouth, NH USA
Jill Veilleux, Corning Inc. Science Products Division, Acton, MA USA
David Dunn, Marc Orlowski, Pharmacopeia, Inc., Princeton, NJ USA
Meng Zhang, Doug Boyd, LJL Biosystems, Inc., Sunnyvale, CA USA
The new Corning 1536–well 2 µl Plate provides the foundation for a unique
solution to assay miniaturization for high throughput screening. The
advantages of the 1536-well HTS plate include:
Conservation of 96 and 384 well format, which aids in sample transfer, well
identification, and data manipulation
Low profile, which provides increased sample density over conventional plate
height and reduces parallax effects in imaging systems
Three point positioning and tight manufacturing tolerances for all
dimensions,
which enable high-speed automated plate handling functions
The lowest volume wells (2 µl) that are commercially available
Optimized materials for manufacture, which enhance performance in
Fluorescence,
luminescence and colorimetric detection formats.
Here, we provide an analysis of evaporative loss and automated dispensing at
low volumes. The Corning 1536-well 2 µl Plate is shown to be an ideal
solution to scale up from traditional 96 and 384 well formats in high
throughput
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Posters will also be presented by BioSignal and Perkin Elmer Tropix.
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Directions: (on line directions at http://marriotthotels.com/EWRHO/):
Take Route 287 to Exit 39 (Route 10 West). Go through one stoplight and take
1st u-turn.
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Detailed information may be found at
http//www.lab-robotics.org/Mid_Atlantic/meetings/9912.htm
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The Laboratory Robotics Interest Group San Diego Chapter
Tuesday, December 7th, 630 - 930pm.
Held in Garren Auditorium, Basic Science Building, UCSD Medical School, La
Jolla Campus.
Featuring presentations on the following
Tentative - Robots and DNA Mass Array Qualification - James E.
Kahelin.
Tentative - Discovery, Selection, and Early-Stage Development of Novel
Small Molecule Drug Therapies - Kia Motesharei, Ph.D., Manager, New
Technologies, Trega Biosciences, Inc.
Automated Synthesizer utilizing Tilted Plate Centrifugation - Michal
Lebl, Spyder Instruments, Inc.
Detailed information may be found at
http//www.lab-robotics.org/San_Diego/
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The Laboratory Robotics Interest Group Bay Area Chapter
Tuesday, December 7th, 630 - 930pm.
Detailed information may be found at
http://www.lab-robotics.org/Bay_Area/
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The Laboratory Robotics Interest Group New England Chapter
Next meeting is March 8, 2000
The topic is HTS: A Reality Check
Detailed information will be posted shortly to
http//lab-robotics.org/New_England/index.html
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The next meeting of the SouthEast Chapter of the Laboratory Robotics
Interest Group (LRIG) is set for Friday, February 4, 2000 from 12 noon
to 6 p.m. at the Sheraton Imperial in Research Triangle Park, North
Carolina. The meeting will consist of a vendor show and vendor
presentations.
Detailed information may be found at
http://www.lab-robotics.org/SouthEast/Feb4_2000.htm
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The Laboratory Robotics Interest Group is a rapidly growing special interest
group focused on robotics applications in the laboratory. Our membership
consists of over 5,000 scientists and engineers worldwide.
We are a non-profit organization run by unpaid volunteers for the benefit of
the Laboratory Automation Community. Please visit our web site at
http//www.lab-robotics.org/
