Dr Antony Halsall wrote:
>> Hi all,
>> I am looking for a luciferase reporter plasmid to allow the cloning of a
> putative promoter from a PCR mixture. I would like to make use of a T/A
> cloning system. I am currently trying to make a T/A cloning vector
> using pGL3-Basic from Promega (the MCS contains a Sma I cut site). Does
> anyone know of a commercially available luciferase reporter plasmid that
> is either provided as a T/A cloning vector; contains an EcoRV
> restriction site in the MCS; or contains 2 XcmI, HphI or AspE1
> restiction sites in the MCS?
>> Thanks for any help
>> Dr Antony Halsall
> Dept Psychological Medicine
> UWCM
Dear Dr Halsall,
Try Reporter Gene Assay 'CATalyse Assay' from Intergen (catno: 9359). I
have found it suberb for my applications.
Dr Paul Beckwith
University of Glasgow
