In article <37084331.379FA798 at gaebyok.wonkwang.ac.kr>,
skpark at gaebyok.wonkwang.ac.kr wrote:
> Dear everyone
> I think, if I use carbonate buffer for protein purification, I can
> directly coat the fractions to ELISA plate for testing antigenicity of
> each fractions, because the buffer is usually used for plate coating. If
> you know or have any experience on it, I would appreciate for your
> comments. Thanks.
> sincerely,
> Park, Seung Kyu, M. S.
You don't *have* to use carbonate buffer for coating
ELISA plates (I usually use PBS for diluting my samples).
The optimum buffer will probably depend on your antigen
and on the type of plate that you are coating. It sounds
like your purified fractions should be fine but you may
want to do a quick test with some authentic protein.
Bernard
--
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA
