In article <36267150.3A4EDDCD at julian.uwo.ca>, Dave O'Neill
<doneill2 at julian.uwo.ca> wrote:
> HI,
> Due to technical problems (received in sample buffer) I was unable to
> quantify the total protein content of my cultured P19 (embryonic stem
> cells) cells. I ran equal volumes on a mini-gel and then stained them
> with commassie blue.
If you have any sample left you could try and quantify the protein
after precipitation with TCA and then a wash (TCA and/or acetone or
methanol) to get rid of interfering substances. You would only need
eg. 25 ug to do this if you were careful.
Also, if it was a total protein extract in the sample buffer,
you could possibly assay the DNA content which would at least allow
you to normalise for cell number.
Good luck,
Bernard
--
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA
