loss of activity

[Park, Seung Kyu]

I am purifying cysteine protease from liver fluke using Mono Q and then
Superdex 200. The activity was about 10-times reduced after Superdex 200
than after Mono Q when I check it by spectrophotomer. 2 days interval
was occured from Mono Q to Superdex 200 purification. I think it is
because of inactivation. Sample was stored under optimun activity
pH(pH6.0) with 0.1mM EDTA and 1mM DTT. I consider the spoteneous
inactivation rather than proteolysis. I would appreciated if I can get
general troubleshooting from experiencer. Thanks.

sincerely,

--
Park, Seung Kyu, M. Sc.

mailto:skpark at gaebyok.wonkwang.ac.kr
http://210.112.141.58/
Tel : +82-653-850-6768(lab), +82-653-831-0473(APT)
Dept. Parasitol., Won Kwang Univ., 344-2, Sinyong-dong,
Iksan-si, Chunbuk, 570-749, Korea.