Thank you very much for your reply. This is pointing me in the right
direction. Basically we are looking for any sort of vector that will allow
expression of a foreign protein in a mammalian cell to see what happens
in terms of posttranslational modifications. The problem is that we sort
of need a place to start and could use any promoter that will give good
expression.
Thanks again for your suggestions.
Peter Ahnert.
>Okay, I'll bite. When you say
"transformation" do you mean>to stably transfect? or do you mean to
immortalise the cells?>The latter would be a little too specialised for most
companies to>sell (probably) so I can only give you pointers to the>former;
>A little inventiveness searching GenBank (eg. with Entrez) is
>a good start ("vector" is a very helpful keyword).
>For plasmids only you may want to try VectorDB and the URL
>for this is http://vectordb.atcg.com/vectordb/vector.html> I don't think this has a specific section for expression
>vectors but the search engine is nice.
>Since there are a good variety of plasmids out there it would
>be a good idea to pick your promoter and selection marker
>combination and then move on from there. For common promoters
>(SV40, RSV, CMV) you'll find lots of choices whereas others
>(TK, metallothionein, glucocorticoid inducible, etc.) are a
>little more specialised. Similarly, many companies offer
>neomycin or hygromycin selection but others (puromycin,
>histidinol, blasticidin, zeocin etc.) are supplied by a
>limited number of companies.
> For more obscure promoters or selection markers write
>to the groups that published their existence/characterisation.
>If by "non-commercial" you are referring to the vectors
>themselves then pSV2neo (and many deriavtives) was not
>patented by the creators (?Bruce Howard's group).
> I hope that this helps,
> Bernard
>--
>Bernard Murray, PhD
>Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA
