I transformed the plasmid pUC18 I extracted before into
Ecoli DH5? again.
There were some single clonies on the solid media with
AMP. I extacted the plasmid througe regular procedure
but get nothing.
The competent cells I used were lysed once 8 or 9 hours
before I used them. I stored them under -20
centigrade at once. Maybe the plasmid I extracted before
had gotten some wrong though it was normal on argrose gel.
Since there were single clonies coming out, the plasmid
must have been transformed into Ecoli. Why I extracted
nothing if there were no something wrong in the procedure
of extraction.
