Hello, I am looking for an efficient method for purification of adenovirus and
for DNA extraction from purified virions. I need some pure DNA to carry out an
homologous recombination. I have tested among other things the method of
Kanagae and al ("A simple and efficient method for purification of infectious
recombinant adenovirus" in Jpn J. Med. Sci. Biol, 47, 157-166,1994). I think
that this protocol is good but my problem is about DNA extraction, therefore
after purification and dialysis of purified virus. I used a classic protocol
which consists in an incubation with pronase, phenol extraction and ethanol
precipitation. But after phenol extraction, there's no DNA left. I will very
much appreciate if you could give me some informations. Many thanks in
advance.
