Hi,
I'm about start looking at the effects of lipids on my adherent cell cultures.
I have previously used cholesterol solublised in ethanol and sterile
filtered. I now want to use some other lipidsbut need a bit of advice on the
following points.
1) What should I solublise phospholipids in ?
2) What should I solublise gangliosides in ?
3) I want to extract lipid from tissue samples, will a
chloroform/methanol ie Bligh-Dyer extract
all the lipids including the ones mentioned above ?
4) Also once I have extracted my lipids what solvent should
I use to solublise and filter them ?
5) For the above it would be easy to sonicate to form a micellar
suspension but how do you sterilise afterwards and if such a suspension
is made how long will it keep for ?
If you can help me on any of the above points or suggest references
that might I would be most grateful.
Ian Mc
