====PHOSPHORYLATON WITHOUT CHANGING [SALT]=====

[Calvino Ka-wing Cheng]

I was wondering if it is possible to phosphorylate a primer
(using the buffer that comes with it) and then use that same 
reaction mixture in a PCR setup.  The reason I ask this is that
our KCl concentration is quite high when we phosphorylate a
primer and we were wondering if this has any effect on the Taq
Polymerase used.  Could anyone suggest any alternatives or
anything that has worked for them?  (ie. protocols which didn't
change the salt concentration much OR salt concentrations which
have worked for PCR)

Thanks in advance.

Calvino  

--



 -------Calvino Cheng----------------------University of Calgary-----------
    ckwcheng at acs.ucalgary.ca          http://www.ucalgary.ca/~ckwcheng/
 --------------------------------------------------------------------------