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Dear Everyone:
The unit of a enzyme is variable according to the assaying conditions. I
want to use the enzyme in the fixed condition which is not standard or
optimum for the enzyme, so, I may re-check the unit. For that, which
experimental procedures shoud I follow? I basically learned the enzyme
kinetics including Michaelis-Menten equation. But, experimentally, the
Km or Vmax which can be got by change the concentration of substrate is
varied if I change the mass of the enzyme added. Kind answers would be
appreciated.
Thank you.
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Seung Kyu Park
Department of Hygiene,
Miyazaki Medical College, Japan
E-mail : skpark at post.miyazaki-med.ac.jp
Home Page : http://baroque.miyazaki-med.ac.jp
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