IUBio

What's the experimental sequence for determination of unit of enzyme?

Park Seung Kyu skpark at POST.MIYAZAKI-MED.AC.JP
Mon Aug 26 07:48:45 EST 1996


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Dear Everyone:

The unit of a enzyme is variable according to the assaying conditions. I 
want to use the enzyme in the fixed condition which is not standard or 
optimum for the enzyme, so, I may re-check the unit. For that, which 
experimental procedures shoud I follow? I basically learned the enzyme 
kinetics including Michaelis-Menten equation. But, experimentally, the 
Km or Vmax which can be got by change the concentration of substrate is 
varied if I change the mass of the enzyme added. Kind answers would be 
appreciated.

Thank you.

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Seung Kyu Park

Department of Hygiene,
Miyazaki Medical College, Japan

E-mail : skpark at post.miyazaki-med.ac.jp
Home Page : http://baroque.miyazaki-med.ac.jp
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