IUBio

About PCR cycling sequencing...

Park Seung Kyu skpark at POST.MIYAZAKI-MED.AC.JP
Wed Aug 14 21:24:43 EST 1996


Dear everyone:

When I do the sequencing by PCR cyling sequencing method, sometimes I 
could find unexpected mutation on unexpected site. I had heard the PCR 
sometimes give mutant product. So, could I believe the mutated sequence 
is realy mutated one? Kind explanations would be appreciated.

Sincerely,

SK Park



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