IUBio

How to Preserve Semen Samples?

howeird howeird at netcom.com
Thu Jan 20 14:56:23 EST 1994


This is being posted for a friend who does not have usenet access. Please
respond to the email address below:

I am working on a project involving immunocontraception in seals and plan 
on doing a sperm-oocyte binding assay. We are getting grey seal semen 
samples/specimens from Sable Island in the near future. I would like to 
contact other people in this research area to find out more about buffers 
and protocols. The sperm is valuable stuff ( hard to access , and 
expensive) and so I can't have them dying on me.
I will be incubating grey seal oocytes with bound anti-zona pellucida 
antibodies with the grey seal sperm. The more recent information I can 
acquire will help in not screwing up and killing my sperm (and the 
subsequent killing of yours truly by my supervisor). I am aware that 
there is research being done in this area, but I am having trouble finding 
any info in the current literature.
				Thanks kindly,
				Cyril Boudreau
				CMAKO at AC.DAL.CA






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