We are trying to clone and express mycoplasma genes in E coli. Mycoplasma uses UGA to code for tryptophan. Use of opal suppressors help but we are still haveing trouble with the system. What percentage of the proteins are terminated with UGA in E coli? Would a different cloning and expression host be better? What other cloning hosts use UGA as a stop codon less frequently than E coli. We are looking for possible solutions. Any ideas?
Thanks ahead of time.
