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[Arrays] PBMC for gene expression

Sam e_mac_in at yahoo.com
Tue Jul 5 16:08:06 EST 2005

I received this message a while ago about using PBMC for gene expression experiments.  Have any of you done this in the past?  How does it work out?  

------Original Message-------
GCI Access, Global Repository updatesFrom: 
Erick McKesson <bioservices at earthlink.net>Date: 
Tue, 05 Jul 2005 16:12:04 -0500

To: e_mac_in at yahoo.com

 Dear Researcher,

Have you been looking for a way to compare data between normal and disease state patients?  Many scientists interested in gene expression have been using peripheral blood mononuclear cell (PBMC) specimens for these types of research activities.  The problem for some has been finding a source for both disease state and normal PBMCs.

Fortunately, SeraCare Life Sciences, Inc. is able to provide both fresh and cryopreserved PBMCs from normal and disease state donors.  We can also set up custom collections of disease state PBMCs that are available within 24 hours of collection.  All SeraCare specimens are ethically collected and comply with HIPAA regulations.

To further enhance the reliability of your research efforts, SeraCare provides in-depth characterization information with our PBMCs.  This includes demographic and clinical diagnostic information, as well as 29 separate cytokine responses.   These responses include 23 individual peptides for CMV, EBV and Flu, 1 peptide pool (CEF), and 5 antigens (tetanus, mumps, PPD, candida and dust mite).  With the normal state PBMCs we are able to provide between 4 and 5 billion cells per donor!  For disease state PBMCs we can offer 250 million cells per collection.

Cryopreserved PBMCs have been shown to perform as well as fresh cells, making them a replacement for repeated blood collections.  And just like fresh cells, post-thaw viability typically exceeds 90%.  Click Here for information about T-Cell performance with these cells, and here for information about CD4 and CD8 functionality.

If you have any questions please feel free to contact me.  Thank you for your time and good luck in all your projects!

Warm Regards,  

Erick McKesson
Sales Consultant
SeraCare Life Sciences, Inc.
bioservices at earthlink.net
ph 812 219 8184   
fax 918 512 7134 

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