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[Arabidopsis] Independence of Transformation

Martin Kottackal via arab-gen%40net.bio.net (by marttinet from yahoo.com)
Sat Sep 12 00:34:31 EST 2015

Please go through the article 
Female Reproductive Tissues Are the Primary Target ofAgrobacterium-Mediated Transformation by the Arabidopsis Floral-Dip Method1
   - Christine Desfeux, 
   - Steven J. Clough and 
   - Andrew F. Bent   2

   - Martin Kottackal

     On Friday, September 11, 2015 11:19 PM, Judy Callis <jcallis from ucdavis.edu> wrote:

 Great question! 
Fortunately there are studies that answer your question. Below are two
citations, there may be others. The female gametophyte is the site of
transformation, so they are independent. We don't have to do Southern

Ye GN, Stone D, Pang SZ,Creely W, Gonzalez K, Hinchee M. Arabidopsis ovule
is the target for Agrobacterium in planta vacuum infiltration
transformation. Plant J. 1999; 19:249-57.

Bechtold, N, Jaudeau, B, Jolivet S,Maba B, Veson D, Vosin R, Pelletier G
The maternal chromosome set is the target of the T-DNA in the in planta
transformation of Arabidopsis thaliana. Genetics. 2000; 155:1875-87.

Judy Callis

On 9/5/15 10:56 AM, "Joseph Lee Hill Jr" <jlh722 from psu.edu> wrote:

>Hi all,
>Sorry in advance for the somewhat naive question.
>Is it overstepping to claim each individual plant isolated from a floral
>transformation is an independent transgenic line?
>According to work by Clough & Bent (among others), via floral dip, "T-DNA
>insertion events derived from the same plant are generally independent."
>I can
>find no guidelines for independence, though. Some journals require
>independent transgenic lines, but I don't believe they require a Southern
>or sequencing analysis to actually prove independence. Taken together,
>makes me feel that it is generally accepted that, when doing Arabidopsis
>dip, each transformant can just be assumed to be independent. Would that
>be a
>correct assessment?
>Arab-gen mailing list
>Arab-gen from net.bio.net

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