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[Arabidopsis] Re: Arab-gen Digest, Vol 108, Issue 1

Ute Müller via arab-gen%40net.bio.net (by ute.mueller from aura-optik.de)
Tue Apr 1 15:14:32 EST 2014


Dear Gary,

may I propose you an alternative way for harvesting your root samples? It is a technology called Microsampling, which starts from the intact, living object. I shall say that I speak in my own interest, because I am part of a young company that develops microsampling solutions. As I read regularly the Arabidopsis List, I came across your question.
If you would like, let's speak about it, and then look how to make possible a proof of principle. We are sitting in Jena, Germany - but in spite of this distance we could find a solution via co-operation to local researchers. What do you think of it?

Best regards
Ute
--
Dr. Ute Müller
aura optik gmbh
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Am 01.04.2014 um 19:17 schrieb "arab-gen-request from oat.bio.indiana.edu<mailto:arab-gen-request from oat.bio.indiana.edu>" <arab-gen-request from oat.bio.indiana.edu<mailto:arab-gen-request from oat.bio.indiana.edu>>:

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Today's Topics:

 1. laser microdissection of GFP labeled tissues (Stacey, Gary)


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Message: 1
Date: Tue, 1 Apr 2014 00:35:14 +0000
From: "Stacey, Gary" <staceyg from missouri.edu<mailto:staceyg from missouri.edu>>
Subject: [Arabidopsis] laser microdissection of GFP labeled tissues
To: "arab-gen from net.bio.net<mailto:arab-gen from net.bio.net>" <arab-gen from magpie.bio.indiana.edu<mailto:arab-gen from magpie.bio.indiana.edu>>
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  <C79352C8521A814AA59FA513392CBC633F76A5FE from UM-MBX-T03.um.umsystem.edu<mailto:C79352C8521A814AA59FA513392CBC633F76A5FE from UM-MBX-T03.um.umsystem.edu>>
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Colleagues,

We want to do an experiment where we use localized GFP expression as a marker to allow us to identify regions of plant roots to harvest by laser microdissection. However, all of the fixation procedures we try that are compatible with laser microdissection bleach the GFP so it cannot be seen.

Does anyone know of a procedure where the tissue can be fixed for laser microdissection that would retain GFP fluorescence and also RNA integrity?

Thanks for your help.

Gary

Gary Stacey, Ph.D.
Assoc. Director, National Center for Soybean Biotechnology
Divisions of Plant Sciences and Biochemistry
271E Christopher S. Bond Life Sciences Center
University of Missouri
Columbia, MO  65211
Office phone: 573-884-4752
Lab Phone: 573-884-4799
FAX: 573-884-9395
email: staceyg from missouri.edu<mailto:staceyg from missouri.edu><mailto:staceyg from missouri.edu>
LAB WEBSITE: http://www.staceylab.missouri.edu/
NCSB site: http://www.soybiotechcenter.org<http://www.soybiotechcenter.org/>
NSF/DOE Project site: http://www.soyroothair.org




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