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[Arabidopsis] mutant seed germination question

PEETAMBAR DAHAL via arab-gen%40net.bio.net (by pdahal from ucdavis.edu)
Wed Mar 21 17:07:51 EST 2012

This germination issue was discussed in 2009 as well.  I am copying the suggestions below. 

Thank you

Peetambar Dahal
3.  Jerry D. Cohen   	

	 More options May 7 2009, 1:51 pm
Yes, of course, I should have said 'my colleagues who wish to germinate
seeds in darkness' use GA4.  Light + GA is better.  I will add that GA4
is also preferred over the 4+7 mixture, but either is choice is better
than GA3.
Thanks for the chance for clarification, Jerry

- Show quoted text -

Dr. Jerry D. Cohen, Professor, Bailey Endowed Chair
University of Minnesota
Department of Horticultural Science
and Microbial and Plant Genomics Institute
1970 Folwell Ave., 305 Alderman Hall
Saint Paul, MN 55108
Phone 612.624.9212; FAX 612.624.4941
Lab phone 612.624.1218
Email cohen... from tc.umn.edu

-----Original Message-----
From: arab-gen-bounces from oat.bio.indiana.edu [mailto:arab-gen-bounces from oat.bio.indiana.edu] On Behalf Of Smith Chiang
Sent: Wednesday, March 21, 2012 2:11 AM
To: Arab-gen from magpie.bio.indiana.edu
Subject: [Arabidopsis] mutant seed germination question

dear colleagues,

I have some problem in getting the mutant seeds ordered from ABRC germinated. is there a fasible way to solve this? how about trying GA treatment in 1/2xMS medium?
does anyone have any idea?

 thanks for your input.



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