you should use other type of phytoagar, like that from Invitrogen
(Phytoagar) or Caissonlabs (Phytoblend). I also met such problem with
Sigma Phytagel a few years ago. I am using Phytoblend for many
antibioitic selections these years and it is pretty good.
On Fri, Jun 10, 2011 at 11:29 AM, Rashid Aman <rashidaman from hotmail.com> wrote:
>> Hi there..
> I have some trouble with making gentamicin MS plates. I am using 1x MS, 20% sucrose, pH 5.7 and phtytagel for making the plates. But after autoclave when i cool down the bottle and add gentamicin, the gentamicin get precipitated. Could you please give me some suggestions to solve this problem.
>> Rashid aman
> PhD student Gyeongsang National UniversityJinju, South korea
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