I am looking into using Phytagel for growth of Arabidopsis seedlings on
plates. Phytagel requires divalent cations in order to solidify. The sole
divalent cation in the medium I am using is calcium at 1mM. I am concerned
that the Phytagel may significantly deplete the medium of calcium. Does
anyone know how much divalent cation is required for Phytagel to solidify?
I will likely use the compound at 0.5-1.0%.
Tessa Durham Brooks
Department of Biology
1014 Boswell Avenue
Crete, NE 68333