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[Arabidopsis] Re: Misterious T-DNA line

Gabriel León via arab-gen%40net.bio.net (by gleon from unab.cl)
Thu Jan 21 12:20:41 EST 2010

Hi Joao:


There is a high chance that any insertional line carries more that one T-DNA inserted in the genome. In fact, as long as I remember from the Alonso et al., paper on Science about the Salk collection, the claim that 50% of the lines are supposed to carry 2 or more T-DNA inserted in the genome. Try to analyze more seedlings or an allelic line for that gene.


Good luck,





Gabriel León, PhD

Assistant Professor

Center of Plant Biotechnology

Faculty of Biological Sciences

Universidad Andrés Bello | República 217 | 1 piso

Santiago - CHILE  

Oficce: +56-2-770 30 35 | Lab: +56-2-770 30 34




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