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[Arabidopsis] Postdoc opportunity-Madison, WI

Michael Sullivan via arab-gen%40net.bio.net (by mlsulliv from wisc.edu)
Sun Mar 29 11:18:01 EST 2009

My lab at the US Dairy Forage Research Center in Madison, WI has =20
recently been awarded a USDA-CSREES-NRI grant to support our research =20=

on the biochemical pathways responsible for o-diphenol biosynthesis =20
in red clover. This grant includes support for a postdoc position to =20
start IMMEDIATELY. Qualified candidates will have a PhD in a relevant =20=

field of study; hands-on experience in general molecular biology, =20
including PCR, cloning, sequencing, making gene constructs; =20
biochemistry, including characterization of proteins and analyses of =20
enzymatic activities; and/or plant transformation including plant =20
tissue culture and maintenance of transgenic plants. Candidates =20
should also be able to work independently and have good oral and =20
written communication skills. Applicants must be citizens of the =20
United States, a NATO member nation, or a country with which the =20
United States has a FORMAL alliance. Non-US citizens must also have =20
documentation allowing them to work in the United States.

Interested individuals may contact me by e-mail =20
(michael.sullivan from ars.usda.gov).

The US Dairy Forage Research Center is part of the Agricultural =20
Research Service of the US Department of Agriculture and is located =20
on the University of Wisconsin campus in Madison, WI. For more =20
information on the US Dairy Forage Research Center visit our web =20
site: http://ars.usda.gov/mwa/madison/dfrc

Title: Elucidating the Roles of Hydroxycinnamoyl Transferases and p-=20
Coumaroyl 3-Hydroxylases in o-Diphenol Biosynthesis in Red Clover

PD: Sullivan, Michael L.            Institution: US Dairy Forage =20
Research Center, ARS-USDA

Phenylpropanoid o-diphenols accumulate in tissues of many plants =20
functioning as defensive molecules and antioxidants. Red clover =20
accumulates high levels of two o-diphenols, phasalic acid and =20
clovamide. In red clover, post-harvest oxidation of these o-diphenols =20=

to o-quinones by an endogenous polyphenol oxidase (PPO) prevents =20
breakdown of forage protein during storage. Agronomically important =20
forages like alfalfa lack both PPO and o-diphenols. Consequently, =20
breakdown of their protein upon harvest and storage results in =20
economic losses ($100 million/yr) and release of excess nitrogen into =20=

the environment. Understanding how red clover is able to synthesize =20
and accumulate o-diphenols will help in development of forages that =20
take advantage of this natural system of protein protection. Also, =20
because o-diphenols are powerful antioxidants, this research has =20
implications for human and animal nutrition. Preliminary evidence =20
suggests that specific hydroxycinnamoyl transferases (HCTs) and p-=20
coumaroyl 3=92 hydroxylases (C3Hs) play key roles in red clover o-=20
diphenol biosynthesis and accumulation. Specific objectives of this =20
proposal are 1) Identify and isolate red clover gene sequences =20
encoding HCTs; 2) Characterize the HCTs and a C3H with respect to =20
substrate specificity and reaction characteristics; and 3) Establish =20
the relevance of specific HCTs to biosynthesis and accumulation of =20
specific o-diphenols. These objectives will be accomplished using =20
several complementary approaches including biochemistry, genomics, =20
and reverse genetics.

Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)

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