I have a gene which is in the Gateway entry vector pENTR/SD-DTOPO.
My understanding (as a newcomer to Gateway technology) is that this
entry vector can be used to produce C-terminal fusions, but not for N-
terminal fusions. I would like to produce a his-tagged protein, but
a destination vector is only available commercially for the N-
terminal his-tagged fusion.
Does anyone know of a destination vector available for producing the
C-terminal his tagged recombinant using my entry vector and the
commercially available recombinase?
Franklin and Marshall College