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[Arabidopsis] Re: Arab-gen Digest, Vol 39, Issue 8

shahzad anjam via arab-gen%40net.bio.net (by shahzadbio from yahoo.com)
Sat Jul 19 11:31:09 EST 2008

Dear Cooleagues,
I have transgenic wheat plants in hand, varified by PCR. But I am disappointed that when I try its southren from genomic DNA, it never produce results. The southren of the PCR product of the same plants is positive but from genomic DNA, it is not. I am using Non-redioactive Chromogenic Detection kit (Fermentas) for southren. I restrict the genomic DNA with HindIII enzyme. I have also used almost 20 ug DNA in southren.
I am in trouble in this case. I have also performed the physiological assays of plants, and they are showing significantly different results from controll plants, (indicating the presence of gene presence).
Without doing southren I am not able to publish the rsults.
If someone can help me plz. contact met at shahzadbio from yahoo.com

Muhammad Shahzad Anjam 
Biotechnology, Institute of Biotechonology , 
Bahauddin Zakariya University , Multan (www.bzu.edu.pk)
Pakistan . 
Office (Phone) +92(0)61 9210463, +92(0)61 9210071-4, Ext: 3900-3904
Cell Phone: +92-333-8174001
E-mail: shahzadbio from yahoo.com 

----- Original Message ----
From: "arab-gen-request from oat.bio.indiana.edu" <arab-gen-request from oat.bio.indiana.edu>
To: arab-gen from magpie.bio.indiana.edu
Sent: Thursday, July 17, 2008 22:03:38
Subject: Arab-gen Digest, Vol 39, Issue 8

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Today's Topics:

  1. about aux7 (2007203013 from njau.edu.cn)
  2. Reproductive biology conference in Tucson, Arizona,    USA
      (October 17-19, 2008) (Ravi Palanivelu)


Message: 1
Date: Wed, 16 Jul 2008 17:16:13 -0700 (PDT)
From: 2007203013 from njau.edu.cn
Subject: [Arabidopsis] about aux7
To: bionet-genome-arabidopsis from moderators.isc.org
    <3e9ea3cb-a884-44a6-93b1-164feca6c8fa from m73g2000hsh.googlegroups.com>
Content-Type: text/plain; charset=ISO-8859-1

hello  anybody, i want to find a mutant which is insensitive to the
auxin(IAA or GA3),so i find the type called aux7,i want to know the
detail message about it ? thanks very much!


Message: 2
Date: Thu, 17 Jul 2008 00:41:35 -0700 (PDT)
From: Ravi Palanivelu <rpalanivelu from hotmail.com>
Subject: [Arabidopsis] Reproductive biology conference in Tucson,
    Arizona,    USA (October 17-19, 2008)
To: bionet-genome-arabidopsis from net.bio.net
    <92f7c347-f3dc-4173-9312-cb73968df498 from 25g2000hsx.googlegroups.com>
Content-Type: text/plain; charset=ISO-8859-1

Dear colleagues,

We invite you to participate in an upcoming conference in the field
of  plant reproductive biology, "Frontiers in Sexual Plant
Reproduction III" (http://www.cals.arizona.edu/FSPRIII), that will be
held in Tucson, Arizona on October 17-19, 2008 in the Tucson Marriott
University Park.

Early bird registration closes on July 31, 2008. Please register on or
before this date to take advantage of low fees. The registration fees
for the three-day meeting includes food {Breakfast, 10/17 - 10/19;
lunch (10/17- 10/19) & dinner (10/17)}, transportation and visit to
desert museum on 10/18 and banquet dinner in desert museum on 10/18.

Participants are encouraged to submit an abstract and present a
poster. Three faculty judges will identify top three posters for a
cash award of $500 (first prize), $300 (second prize) and $200 (third
prize). The prizes will be announced and awarded during the conference
banquet dinner (October 18th, 2008).

Six major sessions have been planned for the conference:

Male gametophyte development and function,
Female gametophyte development and function,
Endosperm development and imprinting,
Compatible and incompatible pollen-pistil interactions,
Evolution of plant reproduction,
Systems biology of plant reproduction and emerging technologies

Preliminary program and information on registration, abstracts,
lodging and travel is posted at the FSPR III conference website:

We look forward to seeing you in Tucson in October 2008 for an
exciting scientific meeting on plant reproduction.


The organizers:
Dr. Ravi Palanivelu, University of Arizona (rpalaniv from ag.arizona.edu)
Dr. Dmitry Belostotsky, University of Missouri, Kansas City.


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