I am looking for protocols extracting Arabidopsis nuclear proteins with preserved activity.
Ive been successful in getting either by either percoll gradients or by filtration through 20 um mashes. However I am having problem in breaking the nuclei without losing protein activity (I could break it by using 1% SDS, which denatured my proteins). I tried homogenizer, 10% ammonia sulfate, freezing and thawing, but none of them work to me. So I am wondering that could anybody out there who worked with arabidopsis nuclear extract give some idea/suggestion. It will be so appreciated if you can share your experience with me
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