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[Arabidopsis] Postdoctoral position - 454 transcriptome sequencing of Kalanchoe fedtschenkoi

James Hartwell via arab-gen%40net.bio.net (by James.Hartwell from liverpool.ac.uk)
Tue Feb 5 07:39:22 EST 2008




You will join a 3-year BBSRC-funded project =93Sequencing the =20
trancriptome of Kalanchoe fedtschenkoi: a model for Crassulacean acid =20=

metabolism (CAM), embryogenic plantlet formation and the =20
Saxifragales=94. This is a ground-breaking project that will employ the =20=

latest ultra high-throughput pyrosequencing technology (Roche/454 GS =20
Leonardo) to achieve deep sequencing of the transcribed portion of =20
the K. fedtschenkoi genome.


=A328,289 - =A329,139 pa

You will generate cDNA samples for 454 sequencing and undertake =20
digital northern analysis of the 454 data to identify the genes =20
required for the circadian control of CAM, plantlet formation and =20
other unique aspects of K. fedtschenkoi's biology. You will =20
collaborate with a second bioinformatics postdoc to build contigs =20
from the 454 reads, and develop a K. fedtschenkoi EST database to =20
provide access to the ~15 billion base pairs of K. fedtschenkoi DNA =20
sequence that you will be involved in generating. For the CAM-=20
associated genes identified through the 454 sequencing, you will =20
perform detailed circadian transcriptome analysis using a Beckman-=20
Coulter GeXP system to profile the transcript abundance of groups of =20
30 genes simultaneously. CAM-associated regulatory genes will be =20
manipulated by generating transgenic K. fedtschenkoi lines, and you =20
will perform detailed  phenotypic analysis to study the ability of =20
these lines to perform CAM. You will also coordinate the production =20
of a K. fedtschenkoi BAC library, clone BACs for CAM-associated =20
genes, and sequence the BACs using the Roche/454 GS Leonardo.

You should have (or be about to obtain) a PhD with experience in =20
plant molecular biology, biochemistry and/or physiology with =20
preference being given to individuals with experience of analysis of =20
large genomics data sets. Ideally, you will also have experience of =20
generating plant binary constructs for RNAi and overexpression, and =20
working with non-model plant species. Job Ref: R-567182


=A320,458 - =A322,332 pa

You will be responsible for the growth and maintenance of K. =20
fedtschenkoi, isolation of RNA and DNA samples, generating transgenic =20=

lines to manipulate key CAM genes, and the study of the phenotypes of =20=

these lines.

You should be qualified to BTEC Higher level (or equivalent) with =20
experience in plant molecular biology, biochemistry and/or physiology =20=

with preference being given to individuals with experience of tissue =20
culture based plant transformation. Ideally, you will also have =20
experience of PCR, RT-PCR, and enzyme and metabolite assays. Job Ref: =20=


Closing date for both posts: 22 February 2008

For informal enquiries, please contact Dr. James Hartwell, e-mail: =20
hartwell from liv.ac.uk

Weblink for Hartwell lab research interests: http://www.liv.ac.uk/=20

For full details, or to request an application pack,

visit www.liv.ac.uk/working/job_vacancies/

or e-mail jobs from liv.ac.uk

Tel 0151 794 2210 (24 hr answerphone),

please quote Ref: in all enquiries

Dr. James Hartwell,
Lecturer in Plant Metabolism,
School of Biological Sciences,
University of Liverpool,
Crown Street,
L69 7ZB
United Kingdom
Tel: +44 (0)151 795 4561
Fax: +44 (0)151 795 4410
E-mail: hartwell from liv.ac.uk
Web: http://www.liv.ac.uk/biolsci/people/academic/hartwell_j/index.html

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