I was wondering which primer targeting the Ds-insertion should be used to amplify and verify the insertion in exon trap lines listed as GT_5_xxxxx, e.g. at signal.salk.edu. The lines are part of a collection of exon trap lines generated by an European consortium, but they also may disrupt the target genes.
We got a few lines from the stock center, but a primer that we used for other Ds-insertions did not work.
Thanks for any advice,
GSF Research Center