POSTDOCTORAL RESEARCH POSITION
REGULATION OF PROTEIN PHOSPHATASE 2A ACTIVITY
Laboratory of Alison DeLong
Brown University, Providence Rhode Island
A postdoctoral position is available for a period
of up to three years to study the regulation of
Arabidopsis protein phosphatase 2A (PP2A) by its
scaffolding/regulatory A subunits. PP2A is an
abundant heterotrimeric serine/threonine protein
phosphatase with broad substrate specificity and
a diverse repertoire of cellular functions. The
RCN1 gene and its paralogs PP2AA2 and PP2AA3
encode the three regulatory A subunits, and loss
of RCN1 function leads to reduced PP2A activity,
alterations in polar auxin transport and
pleiotropic effects on plant growth. Mutations
in PP2AA2 and PP2AA3 have little effect on
overall PP2A activity, but functions of the
isoforms encoded by these genes are unmasked in
the rcn1 mutant background. Currently we are
defining the molecular basis for functional
specialization of A subunit isoforms,
characterizing the composition of PP2A complexes
in mutant and wild-type plants, and taking a
phosphoproteomic approach to identification of
Candidates must have a strong background in
molecular genetics and/or biochemistry. To
apply, please send your current CV, a letter
outlining your research interests and
accomplishments, and arrange to have three
letters of reference sent directly to Prof.
Alison DeLong; Dept. of Molecular Biology, Cell
Biology and Biochemistry; Brown University;
Providence RI 02912; TEL 401-863-3888; FAX
401-863-2421. Alison_DeLong at brown.edu
For additional information see:
Zhou, H.W., C. Nussbaumer, Y. Chao and A DeLong
(2004) Disparate roles for the regulatory A
subunit isoforms in Arabidopsis protein
phosphatase 2A. The Plant Cell 16:709-722.
Rashotte, A. M., A. DeLong and G. K. Muday (2001)
Genetic and chemical reductions in protein
phosphatase activity alter auxin transport,
gravity response and lateral root growth. The
Plant Cell 13: 1683-1697.
Deruère, J., K. Jackson, C. Garbers, D. Söll and
A. DeLong (1999) The RCN1-encoded A subunit of
protein phosphatase 2A increases phosphatase
activity in vivo. The Plant Journal 20: 389-399.
Lizotte, D., D. D. McManus, H. R. Cohen, and A.
DeLong (1999) Functional expression of human and
Arabidopsis protein phosphatase 2A in
Saccharomyces cerevisiae and isolation of
dominant-defective mutants. Gene 234: 35-44.
Alison DeLong, Ph.D.
Dept of Molecular Biology, Cell Biology and Biochemistry
Providence RI 02912