We are pleased to announce the release of this plant transformation
vector, pKYLX-myc9-loxP, to the ABRC at Ohio State University.
The vector, used in recent paper from our lab (Guo, H., and Ecker,
J.R. (2003). Plant responses to ethylene gas are mediated by
SCF(EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor.
Cell 115, 667-677.) can be recombined in vitro to rapidly create
myc fusions to any of the 12000 SSP ORFs (http:/signal.salk.edu), by
using Cre Recombinase.
A variety of other vectors for 2-hyb, GST- and His-tag in E. coli,
as well as HA, Flag tag etc. are available from Dr. Steve Elledge
Lab (selledge at genetics.med.harvard.edu) with no MTA need. In
addition, a GST-CRE plasmid is also available to make a life time
supply of the Cre Recombinase enzyme in a 1 step purification method.
Please contact ABRC at Ohio State University for the pKYLX-myc9-loxp
plasmid and its map.
Hai Li, Ph.D.
Joseph R. Ecker Lab
Plant Biology Laboratory
Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla, CA 92037