I'm working on detecting a low abundant protein which is suspected to
work as a transcription factor.
It's been faintly detected only in overexpression line and failed to
detect in Col.
If there is anyone who tried to detect it using immunohistochemistry,
please give me your valuable comments about what the better 2nd
antibody conjugate is.
I read that using enzyme-linked 2nd antibody is better in sensitivity
than using fluorescent dye conjugates.
please tell me your experiences.