Dear Arabidopsis News Group readers,
I was trying to map an Arabidopsis mutation that causes
a change of mobility of 3 isoperoxidases. I found a
RAPD-marker, linked with the mutation. After several
reamplifications, I cloned the polymorphic DNA fragment
(2kb) and sequenced the ends of this fragment
(300 bp from each end).
To my surprise, both 300 bp sequences did not demonstrate
any significant homology to Arabidopsis genome DNA.
There was no homology to microorganism genomes or other
genomes as well.
My mutant was obtained by mutagenesis of ecotype Dijon.
Can anybody tell me where I can get the information about
genome sequence of Dijon?
I also would like to know, if it could be so, if ecotypes
of the one and the same species demonstrate differences over 300+300 bp
Or - what kind of mistakes could lead to this strange result?
Here are my sequences:
Thank you very much for you help in advance.
PhD student Olga Lebedeva.
Department of Genetics
Moscow State University
OLebedeva at newmail.ru
www.newmail.ru -- =D7"=89=AB=9F=A1 =DE'=A6-'=A6 =91=A6=D7=A6=89.