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TAIL-PCR primer design cloning Ecker and Alonso T-DNA lines

vijay at ust.hk vijay at ust.hk
Mon Sep 2 04:29:48 EST 2002

>Hi :
               I'm a grad. student working now to clone a T-DNA 
insertion mutant of Cis-cinnamic acid.The mutant is isolated from 
Ecker and Alonso insertional line CS75100.I would like to design the 
primers for both Left and Right borders of the vector pROK2 for 

Would anyone  please suggest me the three  primers for the TAIL 
reactions or the protocol from their own lab.

Here is the vector and its sequence.

Vector  - pROK2 -  http://signal.salk.edu/tdna_protocols.html
Vector sequence -  http://signal.salk.edu/pBIN-pROK2.txt

Thank you very much for your attention and response despite your busy schedule.




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