I'm a grad. student working now to clone a T-DNA
insertion mutant of Cis-cinnamic acid.The mutant is isolated from
Ecker and Alonso insertional line CS75100.I would like to design the
primers for both Left and Right borders of the vector pROK2 for
Would anyone please suggest me the three primers for the TAIL
reactions or the protocol from their own lab.
Here is the vector and its sequence.
Vector - pROK2 - http://signal.salk.edu/tdna_protocols.html
Vector sequence - http://signal.salk.edu/pBIN-pROK2.txt
Thank you very much for your attention and response despite your busy schedule.