I have the mutant of Arabidopsis thaliana (we call it spy 2) which
has light-green leaves, high elongation of stem like constitutive
GA-response mutant, spy-1. According to Jacobsen S.E., Olszewski N.
E., 1993, spy-1 mutant is characterized by increasing resistance to
inhibitor of GA biosynthesis, paclobutrazol (pcb), compared with
However, when I compared the resistance of my mutant and spy-1 mutant
to pcb, the resistance of spy-1 mutant was not differed from ecotype
Columbia. I dissolved pcb in ethanol, but authors dissolved pcb in
acetone. I would like to know, if the differences in results could be
due to the type of solvent?
Thanks you in advance
PhD student Olga Sklyarova
Department of Genetics
Moscow State University
Trinity2001 at newmail.ru
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