<x-flowed>I've found the method described in the paper by Chang et al. (1993)
"A simple and efficient method for isolating RNA from pine trees"
Plant Molecular Biology Reporter 11:113-116 to work well for a wide
variety of problematic plant tissues.
>>>> <x-charset iso-8859-1>Good afternoon, everyone.
>>>> Recently I made a second attempt at harvesting total RNA from
>> using TRIzol reagent and Invitrogen's protocol, followed by a
>> precipitation. Analysis indicates the only thing I was able to
>> tRNA. Can anyone share tips or a better protocol for extracting total RNA
>> from samples such as siliques?
>>>> I appreciate!
>> Julia Redman
>> The Institute for Genomic Research
>> 9712 Medical Center Dr.
>> Rockville, MD 20850
>>>> To have me paged at TIGR: 301-838-0200
Michael L. Sullivan, Ph.D
Research Plant Molecular
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax