David is correct. We have been using Qiagen's RNeasy to extract RNA from a
whole variety of Arabidopsis tissues for the last few months-- roots,
leaves, apical leaves, and even hypocotyls. We have gotten very consistent,
results with the kit. It is worth the money. I would also invest in RNA
an anti-RNase reagent from Ambion. We do no extractions w/o RNA Secure
It's wonderful because it is formulated to not interfere with other
reactions and can be left in as insurance for procedures like extraction or
At 03:34 PM 12/13/2002 +0000, Dave Yoder wrote:
>I have been using Qiagen's Plant RNeasy columns to extract total RNA from
>day old plate grown Arabidopsis shoots. I have not specifically tried to
>isolate total RNA from silique tissue. Hovever, in a Northern blot
>between total RNA isolated using RNeasy columns and TRIzol method, the RNeasy
>method gives me consistant RNA yeilds of 2-3.5 ug/ul with no degradation.
>total RNA isolated using TRIzol was almost completely degraded. I
>that you try the Qiagen Plant RNeasy kit as it is what other labs here at MSU
>are using to prepare total RNA for microarray analysis.
>Dr. David Yoder
>Department of Plant Biology
>166 Plant Biology Building
>Michigan State University
>East Lansing, MI 48824-1312
>>"Redman, Julia" wrote:
>>> <x-charset iso-8859-1>Good afternoon, everyone.
>>>> Recently I made a second attempt at harvesting total RNA from silique
>> using TRIzol reagent and Invitrogen's protocol, followed by a
>> precipitation. Analysis indicates the only thing I was able to recover
>> tRNA. Can anyone share tips or a better protocol for extracting total RNA
>> from samples such as siliques?
>>>> I appreciate!
>> Julia Redman
>> The Institute for Genomic Research
>> 9712 Medical Center Dr.
>> Rockville, MD 20850
>>>> To have me paged at TIGR: 301-838-0200