I have been using Qiagen's Plant RNeasy columns to extract total RNA from 8-10
day old plate grown Arabidopsis shoots. I have not specifically tried to
isolate total RNA from silique tissue. Hovever, in a Northern blot comparison
between total RNA isolated using RNeasy columns and TRIzol method, the RNeasy
method gives me consistant RNA yeilds of 2-3.5 ug/ul with no degradation. The
total RNA isolated using TRIzol was almost completely degraded. I
that you try the Qiagen Plant RNeasy kit as it is what other labs here at MSU
are using to prepare total RNA for microarray analysis.
Dr. David Yoder
Department of Plant Biology
166 Plant Biology Building
Michigan State University
East Lansing, MI 48824-1312
"Redman, Julia" wrote:
> <x-charset iso-8859-1>Good afternoon, everyone.
>> Recently I made a second attempt at harvesting total RNA from silique tissue
> using TRIzol reagent and Invitrogen's protocol, followed by a polysaccharide
> precipitation. Analysis indicates the only thing I was able to recover was
> tRNA. Can anyone share tips or a better protocol for extracting total RNA
> from samples such as siliques?
>> I appreciate!
> Julia Redman
> The Institute for Genomic Research
> 9712 Medical Center Dr.
> Rockville, MD 20850
>> To have me paged at TIGR: 301-838-0200