<x-flowed>I used two anti-GUS antibodies (both from Molecular Probes)
polyclonal (rabbit) anti-GUS: worked great for immunofluorescence
microscopy (dilutions 1:400 to 1:1500) but Western Blots were very
dirty, high background, practically unusable.
monoclonal (mouse) anti-GUS: used for Immunofluorescence only (so far),
worked very well. Did not try Westerns yet.
Hope it helps.
Sundar's lab wrote:
> Has anyone had experience of using commercial Gus antibodies (eg
> molecular pobes) for immunolocalization?
>> Advice appreciated!
>> Thanks, Megan Griffith
>megan at ima.org.sg>> ---