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Feldmann lines

Kim Leonie Johnson k.johnson at pgrad.unimelb.edu.au
Fri May 18 00:01:47 EST 2001

I have screened the Feldmann lines by PCR using a gene specific primer and
the LB border primer which resulted in a band being amplified from the
superpool CD5-2.  The band was sequenced and is real so having ordered the
next pools of 20 lines i screened these by the same method.  I have a
positive for only one pool yet no second positive allowing me to determine
which pool of 10 seed lines i require.
Has anyone encountered the same problem and if so have they any
suggestions on how i can find the second pool.  I have tried nesting and
also have blotted the gels without success.  Any help would be
Thanks Kim

Kim Johnson
Plant Cell Biology Research Centre
Botany Department
University of Melbourne
Parkville, 3052
Ph: 03 8344 4870


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